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19th April 2012 @ 04:51
Data obtained for compounds synthesised by Soumya Bhattacharyya and Sanjay Batra. Conclusions: 1) 3-fluoro "near neighbour" derivative is much less active than closely related compounds described here. 2) The IC50 for PMY 10-6 broadly matches the results obtained by other labs, meaning this is a good control to use in conjunction with chloroquine. Data for (3-fluorophenyl) pyrrole derivative SBBH-1-9 (or isomer) [blog]2472[/blog] (Original data posted to G+ (with wrong name) - screenshot attached) 3D7: Sample Code, IC50 (microM), CC50 (microM), SI SBBH-1-9: 3.2, 92.4, 28.87 Chloroquine: 0.0063, 125.85, 19976.2 K1 strain: Sample Code, IC50 (microM,) CC50 (microM), SI SBBH-1-9: >5.0, 92.4, 18.48 Chloroquine: 0.235, 125.85, 535.53 [data]2261[/data] Control compounds sent from Sydney was PMY 10-6 (TCMDC-123812) [blog]2508[/blog], which was evaluated (13/04/2012) vs.chloroquine. Data attached [data]2259[/data] and results pasted here: For 3D7: PMY 10-6 IC50 0.581 microM, CC50 (cytotoxicity) nd, SI (selectivity index) - CQ IC50 0.0083 microM, CC50 125.85, SI 15162.7 For K1: PMY 10-6 IC50 0.641, CC50 nd, SI - CQ IC50 0.252, CC50 125.85, SI 499 Method used for cytotoxicity assay: [data]2263[/data] In more recent correspondence in late 2014, assay protocol was updated by Sanjay Batra: [data]14465[/data]
Attached Files
Original 3-F data
cytotox assay SOP
Text S Assay Protocol Batra New.docx