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1st July 2014 @ 00:23

Adelaide Dennis, from Kiaran Kirk's lab at the ANU, sent data on June 9th by email:

"I have attached my raw data from experiments testing the effect of compounds OSM-S-4/5/35/51/106/111 at 150 µM and 1 µM on [Na+]i of saponin-isolated SBFI-loaded trophozoite stage 3D7 parasites.

Originally sent: Compounds sent for parasiste ion regulation assays at ANU

These compounds were tested at 150 µM and 1 µM. At 150 µM OSM-S-5 appeared to increase [Na+]i within the parasite. At 150 µM OSM-S-4 did not cause an increase in [Na+]i, and the remaining four compounds (35, 51, 106, 111) caused an 'optical effect'. In this assay, excitation wavelengths are 340 nm and 380 nm, while emission is measured at 520 nm. An 'optical effect' occurs when the addition of a compound causes a disturbance at one of these wavelengths.

At 1 µM OSM-S-4, OSM-S-5 and OSM-S-111 showed no effect on [Na+]i within the parasite, and OSM-S-35, OSM-S-51 and OSM-S-106 showed slight 'optical effects'.

Fluorescence intensity (FI) was calibrated to [Na+]i for the traces showing compounds tested at 150 µM. Fluorescence intensity was not calibrated for traces showing compounds tested at 1 µM."

This assay is used to suggest whether bioactives are targeting PfATP4. This screen was carried out to investigate whether OSM Series 1 or 3 might have such a mechanism of action, as Series 4 appears to. The data suggest they do not.

Compounds assayed:

Preparation of OSM-S-4

Preparation of OSM-S-5

Preparation of OSM-S-35

Preparation of OSM-S-51

Preparation of OSM-S-106

Preparation of OSM-S-111

Adelaide made her original lab book entry available - the team uses Labarchives:

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